DataEntryID 82
    General information
      Manuscript title: Network quantification of EGFR signaling unveils potential for targeted combination therapy.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/23752269
      Journal: Molecular Systems Biology
      Year: 2013
      Authors: Bertram Klinger, Anja Sieber, Raphaela Fritsche-Guenther, Franziska Witzel, Leanne Berry, Dirk Schumacher, Yibing Yan, Pawel Durek1, Mark Merchant, Reinhold Schaefer, Christine Sers and Nils Bluethgen
      Affiliations: Laboratory of Molecular Tumour Pathology, Institute of Pathology, Charité - Universitatsmedizin Berlin, Berlin, Germany, and  Institute for Theoretical Biology, Humboldt University Berlin, Berlin, Germany
      Keywords: cancer, EGFR signaling, modular response analysis, signal transduction
      Full text article: https://www.kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBcU1FIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--42799004e1826364514ce0102e35dd6d54efa2c2/Klinger_2013.pdf
      Project name: not specified

    Experiment description
      Organism: Homo sapiens
      Strain: colorectal cancer cell lines SW480, SW403, HCT116, RKO, LIM1215 and HT29
      Data type: enzyme/protein concentrations
      Data units: (a.u.)
      Execution date: not specified

    Experimental details
      Temperature (°C): 37.0
      pH: not specified
      Carbon source: not specified
      Culture mode: batch
      Process condition: aerobic
      Dilution rate (h⁻¹): —
      Working volume: not specified L
      Biomass concentration (g/L): not specified
      Medium composition: All cell lines were maintained in DMEM (Dulbecco’s modified Eagle’s medium, Lonza) supplemented with 10% fetal calf serum, 1% ultraglutamine and 1% penicillin/streptomycin and incubated in a humidified atmosphere of 5% CO2 in air at 37 ºC.
      General protocol information: Measurement method: immunoblotting;
      Methods description: Immunoblotting - protein extracts of cells were prepared as described for Bioplex analysis. Blotting procedure and materials were as previously described [1,2]. The following primary antibodies were used: rabbit anti-human P-p70S6 (Thr389, Cell Signaling Technology, 1:500) or mouse antihuman GAPDH (Ambion, 1:12 500). Membranes were scanned using Li-COR Odyssey. The signals were quantified using Odyssey software.

-------------------References-------------------
[1] Fritsche-Guenther R, Witzel F, Sieber A, Herr R, Schmidt N, Braun S, Brummer T, Sers C, Bluthgen N (2011). Strong negative feedback from Erk to Raf confers robustness to MAPK signalling. Mol Syst Biol 7: 489. http://doi.org/dvbp78
[2] Stelniec-Klotz I, Legewie S, Tchernitsa O, Witzel F, Klinger B, Sers C, Herzel H, Bluthgen N, Schafer R (2012). Reverse engineering a hierarchical regulatory network downstream of oncogenic KRAS. Mol Syst Biol 8: 601. http://doi.org/f2phtg 
      Data file: http://kimosys.org/repository/82/download?parameter=1186; 
      Alternative formats: no files uploaded

    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2014-04-23 16:07:23 UTC
      Updated: 2020-04-24 16:10:35 UTC
      Version: 0
      Status: (reviewed) 2018-07-07 21:37:41 UTC
      Views: 283
      Downloads: 120