Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations. http://www.ncbi.nlm.nih.gov/pubmed/17379776 Science 2007 Nobuyoshi Ishii, Kenji Nakahigashi, Tomoya Baba, Martin Robert, Tomoyoshi Soga, Akio Kanai, Takashi Hirasawa, Miki Naba, Kenta Hirai, Aminul Hoque, Pei Yee Ho, Yuji Kakazu, Kaori Sugawara, Saori Igarashi, Satoshi Harada, Takeshi Masuda, Naoyuki Sugiyama, Takashi Togashi, Miki Hasegawa, Yuki Takai, Katsuyuki Yugi, Kazuharu Arakawa, Nayuta Iwata, Yoshihiro Toya, Yoichi Nakayama, Takaaki Nishioka, Kazuyuki Shimizu, Hirotada Mori, Masaru Tomita Institute for Advanced Biosciences, Keio University Escheirchia coli, perturbations response, chemostat, single knockouts no file uploaded not specified Escherichia coli WT K-12 BW25113 and mutants metabolites at steady-state mM not specified 37.0 °C 7.0 glucose chemostat aerobic 0.1, 0.2, 0.4, 0.5 and 0.7 h⁻¹ 1.0 L See Supplementary files of the original article (Tabel S3). g/L Synthetic medium: 48mM Na2HPO4, 22mM KH2PO4, 10mM NaCl, 45mM (NH4)2SO4, 4g/L glucose, 1mM MgSO4, 1mg/L thiamin.HCL, 5.6 mg/L CaCl2, 8 mg/L FeCl3, 1 mg/L MnCl2.4H2O, 1.7 mg/L ZnCl2, 0.43 mg/L CuCl2.2H2O, 0.6 mg/L CoCl2.2H2O and 0.6 mg/L Na2MoO4.2H2O Sampling Method: Aliquots of 10 mL were withdrawn at OD600 0.85; Quenching: Cells were filter (0.45-µm), washed with 10 mL of Milli-Q water and plunged into 1 mL of methanol containing 10µL of 0.56 mM PIPES; Extraction list: chloroform; Analysis list: CE-MS; Metabolites were quantified using peak areas obtained from measurements of chemical standards mixtures that were analyzed in parallel with experimental samples. See more details in the original article. http://kimosys.org/repository/41/download?parameter=1130; Administrator KiMoSys 2013-05-02 17:35:25 UTC 2020-04-24 16:10:31 UTC 1 (reviewed) 2013-12-06 17:17:38 UTC 675 63