DataEntryID 110
    General information
      Manuscript title: Metabolic flux analysis for a ppc mutant Escherichia coli based on 13C-labelling experiments together with enzyme activity assays and intracellular metabolite measurements.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/15158257
      Journal: FEMS Microbiology Letters
      Year: 2004
      Authors: Lifeng Peng, Marcos J. Arauzo-Bravo, Kazuyuki Shimizu
      Affiliations: Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan.
      Keywords: ppc mutant Escherichia coli, 2-Dimensional nuclear magnetic resonance, Gas chromatography-mass spectrometry, Metabolic flux analysis
      Full text article: no file uploaded
      Project name: not specified

    Experiment description
      Organism: Escherichia coli
      Strain: BW25113 and ppc mutant
      Data type: metabolites at steady-state
      Data units: mM
      Execution date: not specified

    Experimental details
      Temperature (°C): 37
      pH: 7.0
      Carbon source: glucose
      Culture mode: chemostat
      Process condition: aerobic
      Dilution rate (h⁻¹): 0.2
      Working volume: 1.0 L
      Biomass concentration (g/L): Yield of biomass on glucose (g/g): 0,48 (WT) and 0,55 (ppc)
      Medium composition: M9 minimal medium as described previously [3].
      General protocol information: Sampling Method: Cells were harvested by centrifugation at 10,000 g for 10 min.; Quenching: Washed twice with 100 mM Tris-HCl (pH 7.0) containing 20 mM KCl, 5 mM MnSO4, 2 mM DTT and 0.1 mM EDTA, and then resuspended in the same buffer (ca. 15 g wet cells in 50 ml buffer solution).; Extraction list: sonication; Analysis list: enzymatic;
      Methods description: Intracellular metabolite concentrations in quenched cell broth were measured by enzymatic procedures using a fluorescence spectrophotometer (F-4010, Hitachi Co.,
Japan) as described by Schaefer et al. [1] and Bergmeyer [2].                                                                                                                                                                                                                                                                                                                        ----------------------------------References-------------------------------------- 
[1] Schaefer, U., Boos, W., Takors, R. and Weuster-Botz, D. (1999). Anal. Biochem. 270, 88–96. http://doi.org/dt38q9
[2] Bergmeyer, H.U. (1984) Methods of Enzymatic Analysis, 3rd edn,Vol. VI. Metabolites 1: Carbohydrates, pp. 220–227. VCH, Weinheim, Germany.
[3] Zhao, J. and Shimizu, K. (2002). J. Biotechnol. 101, 101–117. http://doi.org/cdjhrn
      Data file: http://kimosys.org/repository/110/download?parameter=1233; 
      Alternative formats: no files uploaded

    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2018-07-29 10:54:27 UTC
      Updated: 2020-04-24 16:10:36 UTC
      Version: 1
      Status: (reviewed) 2018-07-29 10:55:38 UTC
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