DataEntryID 101
    General information
      Manuscript title: Effect of a single-gene knockout on the metabolic regulation in Escherichia coli for D-lactate production under microaerobic condition.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/15781419
      Journal: Metabolic Engineering
      Year: 2005
      Authors: Jiangfeng Zhu, Kazuyuki Shimizu
      Affiliations: Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan
      Keywords: Escherichia coli, Enzyme activity, Metabolite concentration, Metabolic flux analysis, Lactate production, Pyruvate metabolism
      Full text article: https://www.kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBc0lFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--cdd825bd19e8de2977f948f20694a2a489dc69ca/Zhu_2005.pdf
      Project name: not specified

    Experiment description
      Organism: Escherichia coli
      Strain: BW25113, pflA, pta, ppc, adhE and pykF mutants
      Data type: metabolites at steady-state
      Data units: yield on glucose (g/g)%
      Execution date: not specified

    Experimental details
      Temperature (°C): 37
      pH: 7.0
      Carbon source: glucose
      Culture mode: batch
      Process condition: aerobic
      Dilution rate (h⁻¹): —
      Working volume: 1.0 L
      Biomass concentration (g/L): See worksheet for the different strains. Cell dry weight was determined by measuring the optical density at 600 nm. 
      Medium composition: Reactor medium was a minimal medium containing (per liter) 2 g of Na2SO4, 2.5 g of (NH4)2SO4, 0.5 g of NH4Cl, 7.3 g of K2HPO4, 3.6 g of NaH2PO4. The following components were sterilized by passing through a 0.2 μm pore-size filter (per liter of final medium): 3 ml of 1 M MgSO4, and 3 ml trace element solution containing (per liter) 0.5 g of CaCl2·H2O, 0.18 g of ZnSO4·7H2O, 0.1 g of MnSO4·H2O, 21.1 g of Na2EDTA, 16.7 g of FeCl3·6H2O, 0.16 g of CuSO4·5H2O and 0.18 g of CoCl2·6H2O. Glucose was used as a carbon source and the initial concentration was about 10 g/L.
      General protocol information: Sampling Method:  Samples were centrifuged for 10 min at 4 °C and 6000×g to remove the cells for extracellular metabolite analysis. ; Quenching: —; Extraction list: not used; Analysis list: enzymatic;
      Methods description: The concentrations of D-lactate, acetate, formate and succinate were measured using capillary electrophoresis (CE) (Agilent, Germany).
      Data file: http://kimosys.org/repository/101/download?parameter=1217; 
      Alternative formats: no files uploaded

    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2018-07-19 14:32:27 UTC
      Updated: 2020-04-24 16:10:36 UTC
      Version: 1
      Status: (reviewed) 2018-07-19 14:34:40 UTC
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      Downloads: 74